Effect of Artificial dsRNA on Infection of Pea Plants by Pea seed-borne mosaic virus
نویسندگان
چکیده
Šafářová D., Brázda P., Navrátil M. (2014): Effect of artificial dsRNA on infection of pea plants by Pea seedborne mosaic virus. Czech J. Genet. Plant Breed., 50: 105–108. The effect of direct application of artificial dsRNA molecules derived from the viral coat protein gene and the possibility of RNA interference induction leading to protection against Pea seed borne mosaic virus (PSbMV) have been investigated. Effectivity of virus transmission was not afected by the dsRNA treatment. Simultaneous application of dsRNA and virus inoculation as well as dsRNA application one day before PSbMV inoculation led to a significant short-term decrease in the PSbMV concentration in experimental pea plants. Either the curative effect of the artificial dsRNA biolistic application was not noticed.
منابع مشابه
Analysis of the accumulation of Pea enation mosaic virus genomes in seed tissues and lack of evidence for seed transmission in pea (Pisum sativum).
Pea enation mosaic virus (PEMV) is an important virus disease of pea. International movement of commercial pea cultivars and germplasm can be problematic due to uncertainty about seed transmission of the viruses responsible for the disease. Whether PEMV is seedborne was assessed by collecting developing seed from infected plants and determining the relative concentrations of the PEMV-1 and PEMV...
متن کاملCorrection: Narrow Bottlenecks Affect Pea Seedborne Mosaic Virus Populations during Vertical Seed Transmission but not during Leaf Colonization
The effective size of populations (Ne) determines whether selection or genetic drift is the predominant force shaping their genetic structure and evolution. Populations having high Ne adapt faster, as selection acts more intensely, than populations having low Ne, where random effects of genetic drift dominate. Estimating Ne for various steps of plant virus life cycle has been the focus of sever...
متن کاملReactive Oxygen and Nitrogen Species and Hormone Signalling in Systemic Infection of Pea by Pea enation mosaic virus
Kyseláková H., Sedlářová M., Kubala M., Nožková V., Piterková J., Luhová L., Novák O., Ilík P. (2013): Reactive oxygen and nitrogen species and hormone signalling in systemic infection of pea by Pea enation mosaic virus. Plant Protect. Sci., 49: 105–119. The physiological responses of pea plants during 40 days of compatible interaction with Pea enation mosaic virus (PEMV) were evaluated. PEMV i...
متن کاملApple Latent Spherical Virus Vector as Vaccine for the Prevention and Treatment of Mosaic Diseases in Pea, Broad Bean, and Eustoma Plants by Bean Yellow Mosaic Virus
We investigated the protective effects of a viral vector based on an Apple latent spherical virus (ALSV) harboring a segment of the Bean yellow mosaic virus (BYMV) genome against mosaic diseases in pea, broad bean, and eustoma plants caused by BYMV infection. In pea plants pre-inoculated with the ALSV vaccine and challenge inoculated with BYMV expressing green fluorescence protein, BYMV multipl...
متن کاملP1 peptidase of Pea seed-borne mosaic virus contains non- canonical C2H2 zinc finger and may act in a truncated form
Background: Pea seed-borne mosaic virus (PSbMV) is a member of the genus Potyvirus that can be transmitted by seeds, causing serious losses in legume crop yields. Its genome consists of 3 peptidases-P1, HC-Pro, and NIa. Interestingly, only the P1 peptidase, localized on the N-terminal portion of the viral polyprotein, still remains without any concrete function on influencing viral infections. ...
متن کامل